FastQCFastQC Report
Mon 3 Jul 2017
G117-M11_CGTACG_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M11_CGTACG_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20714889
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT579940.2799628808052025TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG79300.041.43649733
CGTACGT80000.041.10142534
GATCGGA84650.039.8824581
CTCGTAT74450.039.73446344
ACCGTAC82650.039.7303732
CGTAATC66950.039.52034438
GTACGTA82750.039.3368335
TCTCGTA69200.038.4167643
ATCGGAA87750.038.349012
TACGTAA84100.038.28694536
ACGTAAT75500.037.8705937
CACCGTA86250.037.86805331
GTCACCG87000.037.7185729
CGTCTGA91200.037.4767416
TCACCGT88650.036.8428630
AATCTCG72300.036.52565841
ACGTCTG94750.036.11902615
ACACGTC95000.036.0702813
TCGGAAG94250.036.007533
ATCTCGT74950.035.35186842
Produced by FastQC (version 0.11.2)