FastQCFastQC Report
Mon 3 Jul 2017
G117-M11_CGTACG_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M11_CGTACG_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20714889
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG626050.30222223252077285Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG73450.042.20644
CGTCGTG79450.040.7512512
GCGTCGT79750.040.68079411
AGCGTCG80250.040.646610
TGGTCGC78000.039.5516942
GATCGGA84450.039.452451
GGTCGCC79200.039.11685643
GTGGTCG80100.039.01197441
ATCGGAA86400.038.6136862
GATCTCG83400.038.44462234
GAGCGTC85700.038.1130569
GTCGTGT86150.037.93950313
TCGTGTA85450.037.81353814
TCGGTGG85700.037.3109538
TCTCGGT86500.037.16940736
CGTGTAG88300.037.04152715
CGGTGGT86050.036.77557839
AGAGCGT90000.036.6342438
TCGGAAG91800.036.511663
ATCTCGG88000.036.43580635
Produced by FastQC (version 0.11.2)