FastQCFastQC Report
Mon 3 Jul 2017
G117-M12_GAGTGG_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M12_GAGTGG_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26616350
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT862220.32394374134695403TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT107650.039.64929244
TCTCGTA100150.039.030343
TATCTCG102750.037.67790641
ATCTCGT105350.036.8945242
CGAGTGG125000.036.6149333
ACGAGTG126250.036.1478832
GTCACGA131600.034.94574429
CACGAGT130950.034.76650231
TCACGAG133850.034.44046830
GATCGGA138500.034.2780761
AGTCACG137750.033.75277728
ATCGGAA141950.033.281522
CGTCTGA146800.032.45103516
ACACGTC150650.031.91370213
TCGGAAG149600.031.755813
ACGTCTG151450.031.70155715
CGGAAGA153750.030.6980464
GGATATC128650.030.29354338
CACGTCT161600.029.73762114
CACACGT168650.028.61188312
Produced by FastQC (version 0.11.2)