FastQCFastQC Report
Mon 3 Jul 2017
G117-M13_ACTGAT_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M13_ACTGAT_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33767812
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG735460.21779912776107613Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG83900.040.8316844
TGGTCGC89650.038.21608742
GTGGTCG91500.037.97495741
GGTCGCC91050.037.69985643
GATCTCG97050.036.5287134
TCGGTGG100800.035.4540838
TCTCGGT100750.035.25325436
CGGTGGT100750.034.99114639
ATCTCGG103400.034.37104435
CTCGGTG108000.032.88671537
CGTCGTG127650.028.41385512
GCGTCGT128550.028.28328111
AGCGTCG128350.028.27581810
GATCGGA132350.027.5829371
GAGCGTC136200.026.646089
ATCGGAA137100.026.6276862
GTCGTGT138750.026.31516313
TCGTGTA138350.026.2323814
GTAGATC139350.025.9426731
CGTGTAG141650.025.76126915
Produced by FastQC (version 0.11.2)