FastQCFastQC Report
Mon 3 Jul 2017
G117-M14_ATTCCT_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M14_ATTCCT_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23201335
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT765250.3298301584801047TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA107150.041.5929761
ATCGGAA109600.040.5569082
CTCGTAT100900.039.98110244
TCTCGTA93500.039.44383243
CGTCTGA115800.039.0117416
TCGGAAG115300.038.74263
ACGTCTG118500.038.19710515
ACACGTC118550.038.01402313
TATCTCG98450.037.57258241
CGGAAGA118450.037.544784
ATCTCGT100500.036.95971742
CACACGT131650.034.44857412
GCACACG131750.034.43912511
CACGTCT132650.034.1225614
GAACTCC160900.028.2135121
CAGTCAC163250.027.12027227
GTCACAT162200.026.82124329
TCTGAAC171850.026.6461618
CCAGTCA165350.026.58961526
TCCAGTC170950.026.2332225
Produced by FastQC (version 0.11.2)