FastQCFastQC Report
Mon 3 Jul 2017
G117-M14_ATTCCT_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M14_ATTCCT_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23201335
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG840420.3622291562101922Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG96850.042.9829544
GCGTCGT102900.042.51419411
AGCGTCG103400.042.4784210
CGTCGTG103050.042.38846612
GATCGGA107050.041.5999681
GGTCGCC100750.041.3430743
TGGTCGC101250.041.0324642
GAGCGTC107050.040.947719
GATCTCG106100.040.52781334
ATCGGAA109850.040.4197582
GTCGTGT109050.040.41953313
GTGGTCG104500.040.28537441
CGTGTAG111200.039.619815
TCGTGTA110800.039.52435314
TCGGAAG112300.039.442153
TCGGTGG109850.039.3250238
AGAGCGT114000.038.721448
TCTCGGT110550.038.69779636
CGGTGGT110750.038.5086939
AAGAGCG115450.038.425627
Produced by FastQC (version 0.11.2)