FastQCFastQC Report
Mon 3 Jul 2017
G117-M15_TGACCA_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M15_TGACCA_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29795251
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3399321.1408932248968133Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG386300.043.4350244
CGTCGTG398500.043.39465312
GCGTCGT398950.043.3013811
AGCGTCG400200.043.2978210
GATCGGA409700.042.9415321
TGGTCGC389450.042.75461242
GAGCGTC405250.042.7311339
GGTCGCC392100.042.66035543
ATCGGAA411300.042.6310542
GTCGTGT408450.042.5316213
GTGGTCG397300.042.460341
TCGTGTA406300.042.42104314
GATCTCG408050.042.33965734
CGTGTAG412300.042.1614915
TCGGAAG418850.042.0438083
TCGGTGG411450.042.02757338
AGAGCGT414050.041.9876258
TCTCGGT411000.041.93430736
ATCTCGG412200.041.9296835
CGGTGGT407750.041.88004339
Produced by FastQC (version 0.11.2)