FastQCFastQC Report
Wed 10 Jun 2015
G117-M1_ATCACG_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M1_ATCACG_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23371443
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1459520.6244886120210892TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA200450.041.9962271
CGTATGC196600.041.71943344
ATCGGAA203250.041.2616842
TCGTATG199250.041.19775843
CTCGTAT183950.041.0414842
TCTCGTA167000.040.8777541
CGATCTC167550.040.56224438
CACGATC167000.040.43242636
CGTCTGA210850.040.27486416
ACGATCT167900.040.22879837
GATCTCG169750.040.1142939
ACACGTC212450.040.0854313
TCACGAT180700.040.00824435
TCGGAAG211600.039.758183
ACGTCTG214500.039.7330915
ATCACGA204900.039.6947334
ATCTCGT171000.039.68951840
CATCACG207400.039.5768133
CGGAAGA214450.038.880844
CACGTCT227050.037.52718714
Produced by FastQC (version 0.11.2)