FastQCFastQC Report
Mon 3 Jul 2017
G117-M2_CGATGT_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M2_CGATGT_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23186862
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3465601.4946395074935108TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA454300.043.130581
ATCGGAA456250.042.7729152
CGTATGC448000.042.3158944
TCGGAAG463250.042.2593423
CGTCTGA460900.042.25521516
ACACGTC464600.042.23587413
TCGTATG449650.042.20474243
TCTCGTA397350.042.19486641
CTCGTAT432350.042.190442
CGATGTA443400.042.11244234
CACCGAT451500.041.9853331
CGGAAGA463700.041.97164
ACGTCTG466650.041.95135515
CCGATGT447400.041.88832533
ACCGATG452350.041.83836732
GTCACCG460750.041.80594629
TATCTCG398700.041.70916439
ATCTCGT401850.041.63457540
CACGTCT474400.041.44219214
TCACCGA461800.041.3060730
Produced by FastQC (version 0.11.2)