FastQCFastQC Report
Mon 3 Jul 2017
G117-M2_CGATGT_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M2_CGATGT_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23186862
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3594691.5503132765442775Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG418350.043.4736844
AGCGTCG434600.043.3980210
GCGTCGT433300.043.38120311
CGTCGTG434400.043.3320212
GATCGGA446450.042.9967541
GTCGTGT439800.042.90027613
TGGTCGC420900.042.89558842
GTGGTCG425700.042.89061441
GGTCGCC423400.042.8380443
GAGCGTC441100.042.7133649
TCGTGTA436900.042.70704314
ATCGGAA448450.042.7031252
GATCTCG442500.042.69421834
CGTGTAG443600.042.48383715
TCGGTGG443850.042.43113338
ATCTCGG445850.042.34938435
TCTCGGT443500.042.28102536
CGGTGGT439050.042.2534539
AGAGCGT449250.042.173498
TCGGAAG455750.042.161613