Basic Statistics
Measure | Value |
---|---|
Filename | G117-M2_CGATGT_BC72YLANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23186862 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 359469 | 1.5503132765442775 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 41835 | 0.0 | 43.47368 | 44 |
AGCGTCG | 43460 | 0.0 | 43.39802 | 10 |
GCGTCGT | 43330 | 0.0 | 43.381203 | 11 |
CGTCGTG | 43440 | 0.0 | 43.33202 | 12 |
GATCGGA | 44645 | 0.0 | 42.996754 | 1 |
GTCGTGT | 43980 | 0.0 | 42.900276 | 13 |
TGGTCGC | 42090 | 0.0 | 42.895588 | 42 |
GTGGTCG | 42570 | 0.0 | 42.890614 | 41 |
GGTCGCC | 42340 | 0.0 | 42.83804 | 43 |
GAGCGTC | 44110 | 0.0 | 42.713364 | 9 |
TCGTGTA | 43690 | 0.0 | 42.707043 | 14 |
ATCGGAA | 44845 | 0.0 | 42.703125 | 2 |
GATCTCG | 44250 | 0.0 | 42.694218 | 34 |
CGTGTAG | 44360 | 0.0 | 42.483837 | 15 |
TCGGTGG | 44385 | 0.0 | 42.431133 | 38 |
ATCTCGG | 44585 | 0.0 | 42.349384 | 35 |
TCTCGGT | 44350 | 0.0 | 42.281025 | 36 |
CGGTGGT | 43905 | 0.0 | 42.25345 | 39 |
AGAGCGT | 44925 | 0.0 | 42.17349 | 8 |
TCGGAAG | 45575 | 0.0 | 42.16161 | 3 |