FastQCFastQC Report
Mon 3 Jul 2017
G117-M4_AGTCAA_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M4_AGTCAA_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28003302
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA35900.032.78954343
CTCGTAT39650.032.63499544
AATCTCG40300.029.15484641
CGTCTGA54000.027.49250216
ATCTCGT44300.026.77115642
ACACGTC57100.026.46213513
ACGTCTG57300.026.13946715
GATCGGA60100.023.6193921
ATCGGAA60200.023.5288852
TCGGAAG67500.021.6033423
CACGTCT71650.021.42611714
CGGAAGA69550.020.7765034
CACACGT77050.019.7532112
GCACACG84300.018.10656711
AGTCAAC86150.016.313634
GTCAACA90300.015.51514735
AACAATC85650.014.0977338
GAACTCC109400.013.871901521
CAGTCAA105900.013.68654133
ACAATCT92650.013.2699939
Produced by FastQC (version 0.11.2)