FastQCFastQC Report
Mon 3 Jul 2017
G117-M7_GTCCGC_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M7_GTCCGC_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13050978
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT526000.40303492964282067TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC72350.041.1312933
ACGTCCG73550.040.5798332
CTCGTAT68100.040.5537944
TCCGCAC74300.040.05180435
GTCCGCA74150.039.86586834
GATCGGA76300.039.7448431
TCTCGTA63250.039.55148743
ATCGGAA77950.038.5155642
CGCACAT69050.038.3509337
CCGCACA77250.038.29454436
ATCTCGT65900.038.12825442
CGTCTGA81100.037.66990316
CATCTCG67000.037.5022741
TCGGAAG81450.037.319433
ACACGTC82250.037.30365813
AGTCACG81250.037.19430528
GTCACGT82000.036.88093629
ACGTCTG83400.036.8420215
CGGAAGA83700.036.0793044
CACGTCT86950.035.38842414
Produced by FastQC (version 0.11.2)