FastQCFastQC Report
Mon 3 Jul 2017
G117-M7_GTCCGC_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M7_GTCCGC_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13050978
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG570850.4374001703167379Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG67250.042.4006844
AGCGTCG71750.041.1704910
CGTCGTG72200.041.00575312
GCGTCGT72300.040.7661911
TGGTCGC69800.040.69857442
GTGGTCG70850.040.53193741
GGTCGCC70450.040.4770743
GATCGGA76250.040.060631
GATCTCG74800.039.68612734
GAGCGTC75700.039.225629
TCGTGTA75400.039.11989614
GTCGTGT76300.039.0907313
TCGGTGG76200.038.8709538
TCTCGGT76000.038.6835436
ATCTCGG76800.038.5098135
ATCGGAA79000.038.4722822
CGGTGGT76400.038.4523639
AGAGCGT78500.038.0786678
CGTGTAG79100.037.8464615
CTCGGTG77650.037.83335537
Produced by FastQC (version 0.11.2)