FastQCFastQC Report
Mon 3 Jul 2017
G117-M8_GTGAAA_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M8_GTGAAA_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24510184
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT279350.1139730325973889TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA50000.036.2266961
CTCGTAT44450.034.7682244
ATCGGAA51950.034.7164042
AACGATC40100.034.4439438
TCTCGTA40950.033.91742743
GATCTCG40700.033.8007641
CGATCTC41950.033.0563540
ACGATCT41950.032.97738639
CGTCTGA55300.032.85129516
TGAAACG52900.032.7202535
GTCACGT55350.031.74875529
ACACGTC57300.031.66626713
AGTCACG56000.031.34096728
TCGGAAG59050.030.951853
ACGTCTG58850.030.57063115
ATCTCGT45100.030.55200642
GAAACGA56500.030.32400536
AAACGAT50600.029.8609637
CGGAAGA64150.028.4563354
CACGTCT69500.026.0759414
Produced by FastQC (version 0.11.2)