FastQCFastQC Report
Wed 10 Jun 2015
G117-M9_GTGGCC_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M9_GTGGCC_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23699696
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT563740.23786803003717855TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA84200.038.9462781
CTCGTAT77800.038.05335244
TCTCGTA71250.037.56041343
ATCGGAA87950.037.211372
CGTCTGA90350.036.49022716
TATCTCG73100.036.33843641
ACACGTC94600.035.1066113
ACGTCTG95550.034.7115315
ATCTCGT76850.034.65130242
TCGGAAG96350.034.560723
ACGTGGC94900.034.04541832
AGTCACG95550.033.9289128
GTCACGT95500.033.87758329
CGTGGCC95900.033.46106333
CGGAAGA100600.032.969054
CACGTCT107150.031.13841614
TCACGTG106400.030.48972530
CACACGT113250.029.73309312
GCACACG116650.028.88531511
CACGTGG113650.028.62212231
Produced by FastQC (version 0.11.2)