FastQCFastQC Report
Wed 10 Jun 2015
G117-M9_GTGGCC_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M9_GTGGCC_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23699696
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG604860.2552184635617267Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG73700.041.22807344
GCGTCGT77300.041.08827611
AGCGTCG78450.040.8220710
CGTCGTG78050.040.7781712
TGGTCGC77900.038.896342
GATCGGA85250.038.5673071
TCGTGTA81800.038.53344314
ATCGGAA86500.037.9087562
GTGGTCG80700.037.7938941
GTCGTGT84500.037.7701113
GGTCGCC80900.037.50672543
GAGCGTC85450.037.4522179
GATCTCG86050.036.62056434
CGTGTAG86950.036.55477515
TCGGTGG88200.035.82813638
TCTCGGT88200.035.72833636
ATCTCGG88250.035.6831635
AGAGCGT91050.035.3419888
CGGTGGT88800.035.09042739
TCGGAAG93850.035.011513
Produced by FastQC (version 0.11.2)