FastQCFastQC Report
Mon 3 Jul 2017
G117C_T1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68662109
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1459710.21259323683168546TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA952050.042.9355161
ATCGGAA959350.042.4732
CGTATGC937850.042.29879844
TCGTATG943400.042.13407543
CTCGTAT900400.042.0824142
TCTCGTA828900.042.02380841
CGTCTGA979800.041.91164416
ACACGTC988250.041.72687513
TCGGAAG980350.041.7246633
ACGTCTG992900.041.52924315
ATCTCGT842500.041.2695640
CGGAAGA989450.041.116114
CGATGTA448700.040.8411334
ACCGATG455700.040.69641532
CACCGAT455800.040.63923331
CCGATGT456000.040.43330433
CACGTCT1021600.040.39484814
GCACACG1031550.040.01800511
CACACGT1035800.039.91538612
TATCTCG412100.039.8678339
Produced by FastQC (version 0.11.2)