FastQCFastQC Report
Mon 3 Jul 2017
G117C_T3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30969260
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC75700.039.77578733
ACGTCCG76650.039.340232
GATCGGA110900.038.7592931
CTCGTAT98850.038.46312744
TCTCGTA90900.038.23842643
TCCGCAC79650.037.52709235
ATCGGAA115150.037.284762
GTCCGCA81450.036.88678734
CGTCTGA119550.036.24331316
CGTCCCG32050.035.75369635
ACACGTC123150.035.18382613
CCGCACA84950.035.00455536
ACGTCTG124050.034.893115
ATCTCGT100200.034.71134642
TCGGAAG126900.034.196443
CGCACAT79550.034.00761437
CGGAAGA131500.032.8828164
CATCTCG80500.032.1169541
AGTCACG95000.032.1117628
GTCACGT96400.031.5769629
Produced by FastQC (version 0.11.2)