FastQCFastQC Report
Mon 3 Jul 2017
G117C_T4_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T4_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48209880
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA136250.037.652771
CTCGTAT121000.037.20187444
ATCGGAA140300.036.448212
TCTCGTA114100.036.0512643
CGTCTGA148450.034.565116
ACACGTC152450.033.6870313
TCGGAAG155550.033.1999363
GTCACGT150650.033.11137829
AGTCACG151000.033.03462628
ACGTCTG155100.032.98383715
ATCTCGT125150.032.8681542
TATCTCG84800.032.0264941
CGGAAGA166150.031.0814344
CACGTCT177400.029.11037414
AACGATC47900.028.97324638
CGTGGCC111700.028.72800333
ACGTGGC112650.028.5638332
TCACGTG175450.028.49373630
CACACGT186250.027.92789312
GCACACG192100.027.06595811
Produced by FastQC (version 0.11.2)