FastQCFastQC Report
Mon 3 Jul 2017
G117C_T6_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T6_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60384162
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT862270.14279737789521696TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT196150.038.9757244
TCTCGTA183150.038.44425243
TATCTCG190300.036.6986241
ATCTCGT196400.035.68226242
ACGAGTG139800.032.66043532
CGAGTGG142400.032.2494533
GTCACGA145150.031.75967629
GATCGGA269000.031.3284971
CACGAGT148750.030.71010631
ATCGGAA277150.030.2832012
TCACGAG152500.030.25780730
CGTCTGA289300.029.36064716
AGTCACG161950.028.84532228
TCGGAAG295450.028.6306883
ACACGTC299850.028.57700513
ACGTCTG301150.028.43903715
CGGAAGA303250.027.7416824
CACGTCT325600.026.35077314
CACACGT339900.025.2680512
GCACACG344550.024.95256411
Produced by FastQC (version 0.11.2)