FastQCFastQC Report
Mon 3 Jul 2017
G120-M1_AGTCAA-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M1_AGTCAA-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21253759
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT5902552.777179321549661TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA722900.043.3497431
ATCGGAA729600.042.8677062
CTCGTAT692900.042.7667944
CGTCTGA732050.042.67914616
TCTCGTA657800.042.4378643
TCGGAAG737800.042.4302253
ACGTCTG738100.042.41924315
AATCTCG654550.042.34507441
ACACGTC740500.042.32890313
CGGAAGA738350.042.147694
ATCTCGT659950.042.14519542
CACGTCT750100.041.8107314
CACACGT756950.041.5753312
GCACACG756550.041.55552311
AGTCAAC752900.040.64336434
GAACTCC778150.040.23693521
GTCAACA758400.040.20848535
AACAATC692000.039.97080638
CAATCTC695400.039.87656440
ACAATCT698700.039.70710839
Produced by FastQC (version 0.11.2)