Basic Statistics
Measure | Value |
---|---|
Filename | G120-M1_AGTCAA-_BC7MKJANXX_L006_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21253759 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 479589 | 2.2564902519126147 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 54540 | 0.0 | 43.552517 | 44 |
GATCGGA | 70900 | 0.0 | 43.32225 | 1 |
ATCGGAA | 70995 | 0.0 | 43.200825 | 2 |
GCGTCGT | 70795 | 0.0 | 43.194237 | 11 |
AGCGTCG | 70790 | 0.0 | 43.19418 | 10 |
CGTCGTG | 70830 | 0.0 | 43.182007 | 12 |
TGGTCGC | 54075 | 0.0 | 43.13684 | 42 |
GGTCGCC | 54870 | 0.0 | 43.11726 | 43 |
GTGGTCG | 55685 | 0.0 | 43.023502 | 41 |
GATCTCG | 59000 | 0.0 | 42.977753 | 34 |
GAGCGTC | 71150 | 0.0 | 42.93863 | 9 |
GTCGTGT | 71515 | 0.0 | 42.871666 | 13 |
CGTGTAG | 71285 | 0.0 | 42.86794 | 15 |
TCGGAAG | 71700 | 0.0 | 42.84573 | 3 |
TCGTGTA | 71365 | 0.0 | 42.810738 | 14 |
ATCTCGG | 60235 | 0.0 | 42.72478 | 35 |
AGAGCGT | 71795 | 0.0 | 42.66624 | 8 |
TCTCGGT | 59090 | 0.0 | 42.607 | 36 |
TCGGTGG | 61225 | 0.0 | 42.594475 | 38 |
AAGAGCG | 71825 | 0.0 | 42.58209 | 7 |