FastQCFastQC Report
Mon 3 Jul 2017
G120-M3_ATGTCA-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M3_ATGTCA-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22786398
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3126481.3720817129587572TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA387250.043.051621
ATCGGAA393550.042.347612
CTCGTAT376250.042.2382444
CGTCTGA398650.041.90239316
TCTCGTA358000.041.87074343
TCGGAAG400900.041.6764763
AATCTCG355750.041.66736641
ACACGTC402300.041.56232513
ACGTCTG402650.041.5536115
ATCTCGT361750.041.27412842
CGGAAGA405100.041.0822224
CACGTCT411750.040.6885914
GCACACG418950.039.97205411
CACACGT421700.039.78069312
GAACTCC444350.037.37549221
ACATGTC441250.037.33470532
CATGTCA449150.036.74661633
GTCACAT455150.036.449329
GAATCTC408050.036.42383640
TCTGAAC459900.036.39763618
Produced by FastQC (version 0.11.2)