FastQCFastQC Report
Mon 26 Sep 2016
G118-M16_TGACCA-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M16_TGACCA-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29684228
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7157622.4112535451486226Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG297200.1001205084397007Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC1882750.071.014459
AGAGCGT1946300.068.821058
AAGAGCG2039700.065.844697
CGGAAGA2338500.057.1286544
CGCCGTA799150.056.9760146-47
GCCGTAT836100.056.15530846-47
CCGTATC839300.055.9128548-49
CGTATCA834250.055.716448-49
TCGGAAG2433950.055.3698733
TCGCCGT800050.055.18280444-45
ATCGGAA2448250.054.745112
GATCTCG1028550.054.4903334-35
GATCGGA2500350.053.801891
GTCGCCG904500.053.31976344-45
TCTCGGT1027850.053.1323436-37
ATCTCGG1054250.053.1224834-35
GTATCAT878000.052.60803650-51
GTGGTCG931750.052.30955540-41
GAAGAGC2591100.052.0116966
GGTCGCC926450.051.716142-43
Produced by FastQC (version 0.11.2)