FastQCFastQC Report
Mon 26 Sep 2016
G118-M20_ACTTGA-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M20_ACTTGA-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27238319
Sequences flagged as poor quality0
Sequence length125
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1724110.6329722476633011No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA1474710.5414100627869143No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG946840.34761322826125945No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC815330.2993319815367461No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC792520.2909577496320533TruSeq Adapter, Index 8 (100% over 50bp)
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG665740.24441302710347138No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG574690.21098585415641838No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC541410.19876777271020285No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC530240.1946669322728763No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC524600.1925963199123999No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG519220.190621161313222No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC499500.18338136064857746No Hit
AGAGCCCCTCGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGA447520.16429795098588865No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC422910.15526288534912894No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG420710.15445519967660265No Hit
ATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAG418790.15375031036239792No Hit
CTACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGAC398920.14645544022008114No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG380800.1398030473172739No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC367740.13500833146127703No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC366580.134582460833945No Hit
GTCGGGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGC346360.12715909524372632No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG344480.1264688911235675No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG340840.1251325384653877No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC334740.12289304637338302No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG331790.12181001331249552No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA326170.11974674354904206No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA321140.1179000803977661No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG315210.11572300038045667No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC302980.11123300230091293No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA300180.11020503871769767No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT296300.10878057489524225No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT289160.10615926775804337No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA288850.10604545750418738No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT282590.10374722463599902No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG281900.10349390503870669No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA280770.1030790483069091No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC275850.10127276943925946No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG273450.10039165779650353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT280000.086.3571857
TGTTACG147500.046.506433
TATGCCG133700.043.29987746-47
TCTCGAC34800.042.571898
CGGCCTC575750.042.214438
ATGCCGT137600.041.8996746-47
GGCCTCC582250.041.8351339
CTCGACT36750.040.6367729
TTACGAC174450.040.415125
GCCGTCT143650.039.90757448-49
GTCGGTA27950.039.8451081
TACGACT194050.039.5222936
TGCCGTC146800.039.09177848-49
GTTACGA175700.038.9751744
CTCGCTA30050.038.8442571
TCGGGCT170350.038.4895029
GGGCGAT258300.038.3412136-37
CTCGTAT153450.036.6599242-43
GCCTCCA348650.035.0350310-11
GCCGGAT51900.033.6213761
Produced by FastQC (version 0.11.2)