FastQCFastQC Report
Mon 26 Sep 2016
G118-M21_GATCAG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M21_GATCAG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23307525
Sequences flagged as poor quality0
Sequence length125
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1315470.5643971206724009No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1063730.4563890846411191Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT753260.32318317796505636No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA600450.25762066113840915No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC463200.19873409982398388No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC376880.16169885047854715No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA359190.15410902702024346No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA330510.14180398819694498No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA316510.13579734441988156No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT310680.13329600633271874No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT304680.13072173042826296No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA300290.12883821855816951No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA295270.1266844077181082No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG287850.1235008865162646No Hit
ATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGC276320.11855398631986878No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT263480.11304503588433348No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC261170.11205393966111804No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC260050.11157340815895296No Hit
ATTGGCTCTAAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGG250650.10754037590863894No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC249560.10707271578599614No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT247400.10614597646039209No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCG241740.10371757619052216No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGG238540.10234462904147909No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC234600.10065418786421981No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG233620.10023372279982538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA263400.063.88234
TGCCAGT264800.063.7018433
CCAGTAG270850.062.0812155
CCTGCCA277650.060.196461
CAGTAGC290950.058.0786976
CTGCCAG305800.054.7913552
TAGCATA311800.054.2905169
GTAGCAT312450.054.2347078
CGTATCA133150.050.406348-49
GCCGTAT136800.049.95302246-47
TCACCCG211750.049.8760263
CCGTATC138150.049.22800448-49
GTATCAT143800.046.5490250-51
CGCCGTA146000.045.35855546-47
CACCCGG252550.041.6299634
ATCATTA177600.039.3483952-53
GGTCGCC183250.038.03778542-43
GTGGTCG188200.037.89093440-41
GATCTCG218900.037.44231834-35
TAGATCT224350.037.36816832-33
Produced by FastQC (version 0.11.2)