FastQCFastQC Report
Mon 26 Sep 2016
G119-M10_GTTTCG-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M10_GTTTCG-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267175
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1173070.5268158174532692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1056920.4746538346242844TruSeq Adapter, Index 21 (97% over 40bp)
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG1045250.469412936306469No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC852330.3827741956489766No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG672460.30199609963994084No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC648300.29114604793827686No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT572270.25700161785228703No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC555300.2493805343515736No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC540330.24265763393874615No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT537670.24146305043185765No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA481890.21641272411071455No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC468920.21058800678577322No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG448440.20139061196581964No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA435300.195489549078408No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA365000.16391841353921185No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT361950.16254868433018557No Hit
ATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCG350230.15728533143517306No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG326430.14659695268932857No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC326400.1465834799430103No Hit
CTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAA311400.13984710678386458No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG290970.13067216654110816No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG289920.13020062041996797No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC273300.12273671895963453No Hit
GAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCC271160.12177566305559642No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG266480.11967391462994294No Hit
TGCCCCGGGCGTGGGGGGGGCGCGCGCCTCGTCCAGCCGCGGCGCGCGCC261610.11748683881094032No Hit
AAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGC242240.1087879356047635No Hit
CCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG238480.10709935139953765No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA233070.10466976614680577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT178250.081.0383157
TCTCGAC64100.060.0506258
ACTCTCG67900.056.952776
CTCGACT68950.056.1717539
CTCTCGA75650.051.0395747
AGTAGCG178250.048.5629127
GTAGCGA181350.047.5687458
CGGTAGT187500.046.6113623
TCGGTAG198700.043.864282
ATCGGTA205050.042.4507451
AGGGGGC333500.041.124851
TAGCGAC214000.041.00629
TTCGTGC25350.039.8971829
GGCCTCC373350.039.311929
ATGTCTC214700.036.965372
GCATAGT225800.036.9135172
TCTGCGA30750.036.6194271
CGGCCTC400300.036.4720548
CGAAGGG115650.036.422411
TAAGCCG170650.035.8208541
Produced by FastQC (version 0.11.2)