FastQCFastQC Report
Mon 26 Sep 2016
G119-M11_CGTACG-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M11_CGTACG-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19477139
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG907010.46567927661244296No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG873840.4486490546686554No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG717040.3681444179250351No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC641450.32933481657650027No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC629220.3230556602794692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT591430.30365342671734286TruSeq Adapter, Index 22 (97% over 40bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG481600.24726424142683376No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT462040.23722169873100973No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC449730.23090146863972166No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC428180.21983721531175598No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT396630.2036387377016717No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA381920.1960862937826752No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT346230.17776224731979373No Hit
TTCGACCGTCTTCTCAGCGCTCCGCCAGGCCGTGGGCCGACCCCGGCGGG344170.1767045971176773No Hit
ATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCG337070.17305929787737306No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG333370.17115963489298916No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC302910.15552078772965577No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA294760.15133639493972909No Hit
GAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCC256400.13164151059352197No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC244640.12560366283775043No Hit
TCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC227560.11683440776389181No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG220360.11313776628076638No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGC219950.11292726308519951No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA218290.11207498185436783No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT216980.11140239847341028No Hit
CCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG216020.1109095129423269No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG210270.10795733398010866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT138500.079.1672747
TCTCGAC49750.054.53088
ACTCTCG50450.054.245896
CTCGACT50400.053.591449
CGAAGGG91800.046.533271
CTCTCGA62950.043.2852447
TAAGCCG158400.040.659091
TAACACG112900.040.2928926-27
CCGGCCT292100.039.900013
GCATAGT228300.038.5678942
GGCATAG240700.036.8557851
TACCGGC314500.036.731971
CGGGTCT181350.036.52979774-75
ATAGTTC239150.036.519584
GGCCTCC307700.036.3883449
TCACACC319250.036.227269
TTCGTGC22050.033.9963349
AGGCCGT83050.033.60326-27
AGGGGGC295400.033.3992651
AGTTCAC262400.033.2837566
Produced by FastQC (version 0.11.2)