FastQCFastQC Report
Mon 26 Sep 2016
G119-M11_CGTACG-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M11_CGTACG-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19477139
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG541980.27826468764226614Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG325170.16694957098165186No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG308880.1585859196260806No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG252650.12971617648772749No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG220770.11334826947633325No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG207430.10649921428398698No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC205780.1056520672774374No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT202510.10397317593718461No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG202170.10379861231159257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAC26150.047.100018
GCGGCCT71600.047.0355037
ACTCTCG30100.042.105076
CTCTCGA33700.037.430737
AGATCGT62600.033.267449
CTCGATC81850.032.0215930-31
GTAGCAT142650.030.449038
TAAGCCG59250.030.4281461
CCGTACG82250.030.4190682-83
GCCAGTA142700.030.229884
GTAGCGA55200.030.1815368
AGTAGCG56150.029.9887947
TAACACG45950.029.52334626-27
TAGCATA148350.029.3595399
CCAGTAG148550.029.0795355
CTCGACT43100.028.8532499
GCCGAGA115600.028.051828
CAGTAGC156150.027.8926876
TCTAACG98900.027.7950867
CGGTAGT60250.027.6517283
Produced by FastQC (version 0.11.2)