FastQCFastQC Report
Mon 26 Sep 2016
G119-M12_GAGTGG-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M12_GAGTGG-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18364863
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG958510.5219260279807151No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG578300.31489480754634547No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG468570.2551448382707783No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC426650.23231864022073023No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC408980.222697005689615No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC384920.20959590060650057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT355350.1934945008846513TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC307530.16745564614339895No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA274160.14928507770518082No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT266620.1451794113574384No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT263480.1434696245760178No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG218200.11881384576623306No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA208650.11361369807114814No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC207970.11324342577453478No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT191720.10439500692164161No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC187800.10226049603528217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT152050.081.893287
TCTCGAC40950.049.8316968
ACTCTCG43100.047.6219646
CTCGACT44150.046.489399
GGCCTCC313400.040.1112339
CGGCCTC322950.038.777728
TGCGGCG342750.036.8326873
CTCTCGA57350.035.9966057
TAGCGTC197400.034.20702776-77
AGTAGCG118150.034.0391277
GTAGCGA118500.034.0398
CCTGCGG380200.033.5324941
AAACCCG186650.033.464905108-109
CGGTAGT123400.033.1694833
TAACACG87150.032.84123226-27
TAGCGAC125100.032.7187429
AGGGGGC245750.031.9026361
TCGGTAG130200.031.6199052
CCGGCCT193250.031.216483
CGTAGCG216300.031.19077374-75
Produced by FastQC (version 0.11.2)