FastQCFastQC Report
Mon 26 Sep 2016
G119-M1_GGCTAC-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M1_GGCTAC-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19035836
Sequences flagged as poor quality0
Sequence length125
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG610380.3206478559701817Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG376900.19799498167561436No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC335230.17610469012235658No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC299840.15751343938874027No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC298680.1569040624220549No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA250640.13166745080174047No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC232510.12214330907242528No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC228370.11996846369132408No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC224280.11781988455878692No Hit
ATTGGCTCTAAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGG223190.11724728034009119No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTC218220.11463641523282717No Hit
GGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGG213110.1119520046295839No Hit
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCG210780.11072799744650037No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG195550.10272729813389861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA84600.045.11717248-49
GCCGTAT85050.044.94841446-47
CGCCGTA83950.044.8286246-47
CCGTATC88400.043.64889548-49
GCCAGTA113700.041.080224
CCAGTAG123600.037.597355
TAGCATA132400.035.5034569
GCGGCCT65400.034.117677
GTAGCAT139450.034.049468
GTATCAT112700.033.89430250-51
GATCTCG130350.033.04784434-35
TGCCAGT144700.032.4846043
ATCTCGG132800.032.48295234-35
GTGGTCG128000.031.46982440-41
CAGTAGC159450.029.479836
TCTCGGT147100.029.14317936-37
TCGGTGG153150.028.49697138-39
GGTCGCC143050.027.70141442-43
TAGATCT160100.027.48289332-33
CTCGGTG157850.027.02652236-37
Produced by FastQC (version 0.11.2)