FastQCFastQC Report
Mon 26 Sep 2016
G119-M5_ATGTCA-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M5_ATGTCA-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850804
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT11948035.730249058981131TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT219070.10506549291816278TruSeq Adapter, Index 15 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1479200.054.71829248-49
GCCGTCT1483900.054.3967650-51
ATGCCGT1492800.054.22377448-49
CGTATGC1500250.054.1962946-47
TCGTATG1510400.054.0603844-45
TGCCGTC1492000.054.02766850-51
CCGTCTT1487400.053.83823852-53
CTCGTAT1474900.053.60689544-45
CGTCTTC1520850.052.7538652-53
GAGCACA3114500.052.5686579
TCTCGTA1439300.052.5493442-43
GTATGCC1557650.052.30035446-47
GCTTGAA1526100.052.10452760-61
AATCTCG1442050.052.02210640-41
ATCTCGT1442700.051.99660542-43
AGAGCAC3163750.051.5754368
TGCTTGA1543950.051.42120460-61
CTTGAAA1575600.050.01537362-63
TTGAAAA1621150.048.62658762-63
CGGAAGA3360250.048.525784
Produced by FastQC (version 0.11.2)