FastQCFastQC Report
Mon 26 Sep 2016
G119-M6_CCGTCC-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M6_CCGTCC-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17672058
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT573430.3244839961480434TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG135200.029.61694148-49
CGTATGC146450.027.72762346-47
TCGTATG148650.027.41619744-45
CTCGTAT143350.026.89421344-45
GCCGTCT163150.024.6524950-51
ATGCCGT165500.024.41032648-49
TGCCGTC166350.024.28556350-51
CCGTCTT167200.023.84150152-53
GTATGCC183900.022.17813746-47
TCTCGTA167450.021.81603442-43
ATCTCGT174300.020.6002542-43
GATCTCG178850.019.97626140-41
CGTCTTC202600.019.92532752-53
CCGATCT178250.019.77647838-39
CCCGATC186500.018.90164838-39
CGATCTC191800.018.5499540-41
GCTTGAA223800.018.225760-61
TGCTTGA238050.017.1596860-61
TCCCGAT215100.017.1212536-37
CGTCCCG233750.016.82460634-35
Produced by FastQC (version 0.11.2)