FastQCFastQC Report
Mon 26 Sep 2016
G119-M6_CCGTCC-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M6_CCGTCC-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17672058
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG534310.30234735535612206Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC33950.038.907331
CGTATCA111850.029.68342448-49
CCGTATC120250.027.65938248-49
CGCCGTA126350.025.92375246-47
GCCGTAT128800.025.73090746-47
TCGCCGT133250.024.6260144-45
GTATCAT144850.022.81819750-51
GTCGCCG157150.022.1302344-45
ATCATTA163300.020.80490352-53
GGTCGCC173950.020.09551842-43
TGGTCGC175100.019.69169442-43
TATCATT171600.019.46920850-51
GTGGTCG181850.019.32067740-41
TCATTAA188200.018.52651652-53
CATTAAA200300.017.55586654-55
TATGCCG38450.016.403173
ATCTCGG226600.016.35851534-35
TCTCGGT224300.016.27425436-37
CGGTGGT223250.016.13758338-39
GATCTCG229750.016.01769334-35
Produced by FastQC (version 0.11.2)