FastQCFastQC Report
Mon 26 Sep 2016
G119-M7_GTCCGC-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M7_GTCCGC-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22314056
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5666482.539421788669886Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA646300.054.04518548-49
CGCCGTA645650.053.46372646-47
CCGTATC654850.053.13057348-49
GCCGTAT665250.052.78294846-47
TCGCCGT651750.052.58447344-45
GTCGCCG708750.051.54557444-45
GGTCGCC727350.050.19062442-43
TGGTCGC731850.049.51209342-43
GTATCAT701450.049.4185350-51
GTGGTCG751850.049.08136740-41
ATCATTA767250.047.514652-53
TCTCGGT831700.046.7478636-37
ATCTCGG843900.046.48097634-35
CGGTGGT818500.046.47678438-39
GATCTCG846250.046.18667234-35
TCGGTGG861650.045.9895838-39
CTCGGTG854650.045.45076436-37
GAGCGTC1889400.045.1848959
TATCATT769150.044.99139450-51
TCATTAA821450.044.857652-53
Produced by FastQC (version 0.11.2)