FastQCFastQC Report
Mon 26 Sep 2016
G119-M8_GTGAAA-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M8_GTGAAA-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20462747
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1421230.6945450676783523Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA210950.041.7162248-49
CCGTATC221300.039.7920848-49
CGCCGTA223050.038.9063546-47
GCCGTAT233450.037.89949446-47
TCGCCGT229000.037.60965744-45
GTCGCCG258050.035.33563644-45
GTATCAT259350.033.95406350-51
GGTCGCC277750.032.81867242-43
TGGTCGC283400.031.93343742-43
ATCATTA290700.031.57158952-53
GTGGTCG294850.031.24829940-41
TATCATT312750.028.26126950-51
TCATTAA334700.027.73225452-53
CGGTGGT347400.027.386438-39
TCTCGGT358850.026.68666636-37
CATTAAA355000.026.28052154-55
TCGGTGG375950.026.06632638-39
ATCTCGG373750.025.94912534-35
GATCTCG374750.025.78461834-35
CTCGGTG382600.025.16227136-37
Produced by FastQC (version 0.11.2)