FastQCFastQC Report
Thu 6 Aug 2015
G118-M10_GAGTGG-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M10_GAGTGG-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782242
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAAGTATGGTTAATGGGAGACAAAGAGAGATCGGAAGAGCACACGTCT299640.7922285247744592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA276290.7304926548856472No Hit
GGTAAGTATGGTTAATGATCTGTGAGCACGGAGACTGTGTCAAGAATTAA230700.6099556823704035No Hit
GGTAAGTATGGTTAATGATCAATGTTCTATCCCAGACCCATCAATTGTGA147650.39037692458599954No Hit
GGTAAGTATGGTTAATGATCAATTTCATTTTAGTCATCAACATTACATCA133040.35174904197034457No Hit
GGTAAGTATGGTTAATGATCTGGCTGTAATCATTTGGAGAGTGGGAATGA124260.328535297318363No Hit
GGTAAGTATGGTTAATGATCCAGCAATACCTCTCCTGGGCATATATCCAG113210.2993198214180901No Hit
GGTAAGTATGGTTAATGATCAAAGAATAATATATAAAACTATTTAAATCT109400.2892464310850548No Hit
GGTAAGTATGGTTAATGATCCTGAGTTTCAACTGGTAAAGAGCCATAGTC108680.2873427982662135No Hit
GGTAAGTATGGTTAATGATCTTTTTACTGTCATTGTATAGTCTGTGGTAT107390.28393212279912283No Hit
GGTAAGTATGGTTAATGATCACTCTTTCCACCCCATCTCCGGAGCCAGGA103500.2736472177084386No Hit
GGTAAGTATGGTTAATGATCAATTCCTCTTTAAACTTCCTAGAAAGAGTA103090.2725632045754873No Hit
GGTAAGTATGGTTAATGATCAACTTTTCCAGAATCCACACACAAGCAGGT102680.27147919144253596No Hit
GGTAAGTATGGTTAATGATCCTTAACCCCATGTATCCTCTCCTACCTTTG91660.24234303357638143No Hit
GGTAAGTATGGTTAATGATCCCATAGAGTAGAACGGTGTTTACAGGAGAC88640.23435835147513034No Hit
GGTAAGTATGGTTAATGATCGCATAGGTAGCTAAAGAGGTGGCAACCCAC88360.23361804982335874No Hit
GGTAAGTATGGTTAATGATCAACTAGGCCATCCTCTGCTCCATATGCAGC87600.2316086596256929No Hit
GGTAAGTATGGTTAATGATCATTAGATACAGATTCAAAACCATAATGTGT87160.23044532845862323No Hit
GGTAAGTATGGTTAATGATCATCTTATGGCCAGATTCAGGAGGCTTGGCT85510.2260828365821119No Hit
GGTAAGTATGGTTAATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA81500.21548065935495403No Hit
GGTAAGTATGGTTAATGATCTCAAAGCACTGTTATTAACTTGGTTGCAGG81490.21545422001024792No Hit
GGTAAGTATGGTTAATGATCTCATTTTCTCCAACATTGTCCTATTATCTC81190.21466103966906402No Hit
GGTAAGTATGGTTAATGATCTCATAGAAAAAAAGTCAAGGTGGATAGATA74950.19816288857243933No Hit
GGTAAGTATGGTTAATGATCTTGACTCAGAACAATATATGTGTGCACACG72810.19250486880532763No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTAGAGAAA69700.1842822326017214No Hit
GGTAAGTATGGTTAATGATCTGGAACAGCATTTCTCAAGTTATATCAATG67360.17809542594048713No Hit
GGTAAGTATGGTTAATGATCAAATCAAAGAAGTAAGCTCCACCTTAGAAA65520.17323058651455936No Hit
GGTAAGTATGGTTAATGATCCAGAGTTCCTGATTCAATAGCTACTAAATA65140.17222589141572645No Hit
GGTAAGTATGGTTAATGATCTAGCATAAAAAAAATCTTCCATAATTTTAT64770.17124763566159967No Hit
GGTAAGTATGGTTAATGATCAATACAGTCCTCTGCCTTTAAAGCAATAGG63090.16680582575096994No Hit
GGTAAGTATGGTTAATGATCATAGGGCTCCCAATGGAGGAGCTAGAGAAA61410.1623640158403402No Hit
GGTAAGTATGGTTAATGATCTGTTCCTTTCTTCGGGGATATTATTTTTTG55930.14787525494138135No Hit
GGTAAGTATGGTTAATGATCCCTGGATATGGCAGTCTCTACATGGTCCAT55220.14599806146724614No Hit
GGTAAGTATGGTTAATGATCCATCCATTTATCTAAGAATTTCATGAATTC50910.1346027038989044No Hit
GGTAAGTATGGTTAATGATCCACTACAGCACTTGGTAACATGCTCACTCA50550.13365088748948376No Hit
GGTAAGTATGGTTAATGATCTGCAACCCTATAGGTGGAACAACATTATGA50120.1325139956671202No Hit
GGTAAGTATGGTTAATGATCCTTGTGTTTGCGAAAAGCAAGTATTTGCTG48810.12905044151061726No Hit
GGTAAGTATGGTTAATGATCTTCCTGCCTTAGTCTCCCAAGTAGTTGCAA48570.12841589723767013No Hit
GGTAAGTATGGTTAATGATCAAAAACTCAGGTGACAGCAGATGCTGGCGA48100.12717324803648206No Hit
GGTAAGTATGGTTAATGATCATGTTAAATATACATGTGTATATGCGTATG47900.12664446114235947No Hit
GGTAAGTATGGTTAATGATCAGTCCAAACTTCTTTCTGCATGTACATAAA47050.12439711684233848No Hit
GGTAAGTATGGTTAATGATCTTCGTTTGCCTCGCAGTTTAAAAACGTAAC46500.12294295288350138No Hit
GGTAAGTATGGTTAATGATCCACAAAATCAGAAGGTACATAAAAGCATAT46060.12177962171643168No Hit
GGTAAGTATGGTTAATGATCGCTGCGAGTTCAAGGCTAGCCTGGTCTATG45780.12103932006466006No Hit
GGTAAGTATGGTTAATGATCAACTTAGAGCTTGGTTCTGAACAAGACCTT45590.12053697251524362No Hit
GGTAAGTATGGTTAATGATCCTGAAGAAATCCAAAACACCATCAGATCCT45190.11947939872699843No Hit
GGTAAGTATGGTTAATGATCCAAAGGTATATGCTATAGTTTAAAAATTAT43990.11630667736226293No Hit
GGTAAGTATGGTTAATGATCATGTCCATTCATAGGCATGTAGAACGCAGC43240.1143237265093032No Hit
GGTAAGTATGGTTAATGATCAACATTGTAAAAATGGCTATCTTGCCAAAA42380.11204994286457609No Hit
GGTAAGTATGGTTAATGATCTTGTGTAAAGATAATATCTACCAGCAAAGG42010.1110716871104493No Hit
GGTAAGTATGGTTAATGATCCCCTGCTTGGCACATACTAAGTGGCTGACA40900.10813691984806896No Hit
GGTAAGTATGGTTAATGATCCTTCTACAAAAGGCTATACTCAACAAAACT40870.10805760181395058No Hit
GGTAAGTATGGTTAATGATCCAATCAATTTAAAAATGAACTTCCACTTGC39570.10462048700215375No Hit
GGTAAGTATGGTTAATGATCTTAATGTGGACATAGTTTTGTTCTTTAGAG39000.10311344435390438No Hit
GGTAAGTATGGTTAATGATCACATATGGAATAGTCATAATCGAACCCATT38340.10136844760329983No Hit
GGTAAGTATGGTTAATGATCCTAAGACCTCTAGTGAGTGGAACACAACTT37830.10002004102328725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT3411150.0118.6926041
GTATGGT3674450.0118.5209966
AGTATGG3559750.0118.517365
TAAGTAT3421300.0118.3770753
AAGTATG3438900.0118.353924
ATGGTTA3684600.0118.32538
TATGGTT3685300.0118.194667
GTAAGTA3435000.0117.8651052
TGGTTAA3740250.0116.566369
GGTTAGT139350.0106.506071
TAGTATG145900.0103.90424
TTAGTAT144000.0103.4958043
TGTATGG144050.0100.162395
GGTATGT123450.097.482631
CGTATGG5050.094.25515
GTTAGTA160300.093.2317052
GGTACGT5250.087.420531
GTACGGT2950.082.692876
AAGTACG2000.080.3198244
GTACGTA5550.079.3261262
Produced by FastQC (version 0.11.2)