FastQCFastQC Report
Thu 6 Aug 2015
G118-M11_ACTGAT-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M11_ACTGAT-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560036
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAAGTATGGTTAATGATCCAAGAAAGAATTATCTCTTCTGTTTAGTTT678941.9071155460225684No Hit
GGTAAGTATGGTTAATGATCTGTGAGCACGGAGACTGTGTCAAGAATTAA324630.9118728012862792No Hit
GGTAAGTATGGTTAATGGGAGACAAAGAGAGATCGGAAGAGCACACGTCT306180.8600474826659056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA255270.7170433107979807No Hit
GGTAAGTATGGTTAATGATCAATGTTCTATCCCAGACCCATCAATTGTGA195590.5494045565831357No Hit
GGTAAGTATGGTTAATGATCAATTCCTCTTTAAACTTCCTAGAAAGAGTA106320.2986486653505751No Hit
GGTAAGTATGGTTAATGATCCAGCAATACCTCTCCTGGGCATATATCCAG105890.2974408123962791No Hit
GGTAAGTATGGTTAATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA100030.280980304693548No Hit
GGTAAGTATGGTTAATGATCTTTTTACTGTCATTGTATAGTCTGTGGTAT92560.25999737081310414No Hit
GGTAAGTATGGTTAATGATCGCATAGGTAGCTAAAGAGGTGGCAACCCAC90500.2542109124739188No Hit
GGTAAGTATGGTTAATGATCATAGGGCTCCCAATGGAGGAGCTAGAGAAA88490.24856490215267485No Hit
GGTAAGTATGGTTAATGATCAACTAGGCCATCCTCTGCTCCATATGCAGC84570.23755377754606974No Hit
GGTAAGTATGGTTAATGATCACTTGAGCTATTTTCTCTTTATTCAGAAAC83190.23367741225088734No Hit
GGTAAGTATGGTTAATGATCAATTTCATTTTAGTCATCAACATTACATCA77220.21690791890868522No Hit
GGTAAGTATGGTTAATGATCACTCTTTCCACCCCATCTCCGGAGCCAGGA75280.21145853581255922No Hit
GGTAAGTATGGTTAATGATCCTCCGGTAGTACATAAAAGCATATTTGGGA75110.21098101255155846No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTAGAGAAA65390.18367791786375195No Hit
GGTAAGTATGGTTAATGATCAACTTTTCCAGAATCCACACACAAGCAGGT64290.18058806146904133No Hit
GGTAAGTATGGTTAATGATCATTAGATACAGATTCAAAACCATAATGTGT64070.1799700901900992No Hit
GGTAAGTATGGTTAATGATCAAAGAATAATATATAAAACTATTTAAATCT63980.17971728375780469No Hit
GGTAAGTATGGTTAATGATCTCATAGAAAAAAAGTCAAGGTGGATAGATA61460.17263870365355855No Hit
GGTAAGTATGGTTAATGATCCACTACAGCACTTGGTAACATGCTCACTCA60890.1710375962490267No Hit
GGTAAGTATGGTTAATGATCCTGAGTTTCAACTGGTAAAGAGCCATAGTC60400.16966120567320106No Hit
GGTAAGTATGGTTAATGATCCACTTAGATTTGACCTTAGTACATAAAAGC60120.16887469677272926No Hit
GGTAAGTATGGTTAATGATCAACAAAACGTTGAGGGTAAGTCACAGTCAT55580.1561220167436509No Hit
GGTAAGTATGGTTAATGATCTACGTTTCTAATTTTATTCTGGTACATAAA53350.14985803514346482No Hit
GGTAAGTATGGTTAATGATCTGGCTGTAATCATTTGGAGAGTGGGAATGA51850.14564459460522308No Hit
GGTAAGTATGGTTAATGATCCTTAACCCCATGTATCCTCTCCTACCTTTG51330.14418393521863263No Hit
GGTAAGTATGGTTAATGATCTAGCATAAAAAAAATCTTCCATAATTTTAT50970.1431727094894546No Hit
GGTAAGTATGGTTAATGATCCCTGGATATGGCAGTCTCTACATGGTCCAT46530.13070092549625903No Hit
GGTAAGTATGGTTAATGATCAATACAGTCCTCTGCCTTTAAAGCAATAGG45490.12777960672307806No Hit
GGTAAGTATGGTTAATGATCAACATTGTAAAAATGGCTATCTTGCCAAAA44740.1256728864539572No Hit
GGTAAGTATGGTTAATGATCACTTCTAAAACTTTAGAATTTATTGTTTTA44100.12387515182430739No Hit
GGTAAGTATGGTTAATGATCCCATAGAGTAGAACGGTGTTTACAGGAGAC42910.12053248899730228No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTTGAGAAA42870.12042013058294915No Hit
GGTAAGTATGGTTAATGATCCTGAAGAAATCCAAAACACCATCAGATCCT41670.11704937815235575No Hit
GGTTAGTATGGTTAATGATCCAAGAAAGAATTATCTCTTCTGTTTAGTTT41340.11612242123394258No Hit
GGTAAGTATGGTTAATGATCACATATGGAATAGTCATAATCGAACCCATT40890.11485838907247005No Hit
GGTAAGTATGGTTAATGATCCACAAAATCAGAAGGTACATAAAAGCATAT40300.11320110246076164No Hit
GGTAAGTATGGTTAATGATCATAGTATCCTAGTCCACAGGTTCTGCAGCA39870.11199324950646568No Hit
GGTAAGTATGGTTAATGATCCAGAGTTCCTGATTCAATAGCTACTAAATA39810.11182471188493599No Hit
GGTAAGTATGGTTAATGATCTTCGTTTGCCTCGCAGTTTAAAAACGTAAC38900.10926855795840268No Hit
GGTAAGTATGGTTAATGATCCAGAGAACATGAACACCACACACATACTCA38810.10901575152610816No Hit
GGTAAGTATGGTTAATGATCTCCTTTTGTTGTCGAATTGCTCTGGCTAAT38480.10808879460769498No Hit
GGTAAGTATGGTTAATGATCCTGATTCAACTTTGGTACATAAAAGCATAT37930.10654386641033968No Hit
GGTAAGTATGGTTAATGATCCATCCCATAAACAACCACCAAACCCAGACA36640.10292030754745177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT3358350.0118.507066
GGTAAGT3070950.0118.4447861
AAGTATG3093400.0118.2783664
ATGGTTA3371100.0118.27778
AGTATGG3240150.0118.251145
TATGGTT3367050.0118.222057
TAAGTAT3077750.0118.168453
GTAAGTA3096800.0117.508782
TGGTTAA3406900.0117.001659
GGTTAGT159300.0108.44971
TGTATGG148450.0106.9677665
TAGTATG164550.0106.729574
TTAGTAT164850.0105.163953
GGTATGT133300.0100.911321
GGTACGT4900.093.613991
GTACGGT2950.090.761046
GTTAGTA193050.089.9560852
AGTACGG3250.086.0397345
GTACGTA5450.080.7795642
TACGTAT5450.079.687943
Produced by FastQC (version 0.11.2)