Basic Statistics
Measure | Value |
---|---|
Filename | G118-M11_ACTGAT-_AC6PMVANXX_L003_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3560036 |
Sequences flagged as poor quality | 0 |
Sequence length | 125 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA | 3056922 | 85.86772718028695 | No Hit |
CTCTTTGTCTCCCATTAACCATACTTACCAGATCGGAAGAGCGTCGTGTA | 33362 | 0.9371253549121413 | Illumina Single End PCR Primer 1 (100% over 21bp) |
CTCTTTGTCTCCCATTTTAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA | 5297 | 0.14879063020711028 | No Hit |
CTCTTTGTCTCCCCTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA | 4836 | 0.13584132295291396 | No Hit |
CTCTTTTTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA | 4199 | 0.11794824546718066 | No Hit |
CTCTTTGTCTCCCGTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA | 3944 | 0.1107853965521697 | No Hit |
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGCAGCA | 3599 | 0.10109448331421368 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCTCCC | 332305 | 0.0 | 118.89078 | 7 |
TGTCTCC | 333680 | 0.0 | 118.86805 | 6 |
CTTTGTC | 335235 | 0.0 | 118.863335 | 3 |
TCTTTGT | 336380 | 0.0 | 118.85143 | 2 |
TTGTCTC | 335030 | 0.0 | 118.84727 | 5 |
CTCCCAT | 331275 | 0.0 | 118.84015 | 9 |
TCTCCCA | 331490 | 0.0 | 118.82948 | 8 |
CTCTTTG | 335525 | 0.0 | 118.81202 | 1 |
TTTGTCT | 336400 | 0.0 | 118.697556 | 4 |
GTCTCCT | 755 | 0.0 | 106.39073 | 7 |
TCTCGCA | 310 | 0.0 | 105.56451 | 8 |
GTATCCC | 485 | 0.0 | 104.27836 | 7 |
CTCCCGT | 820 | 0.0 | 102.310974 | 9 |
TGTCTCG | 350 | 0.0 | 101.99999 | 6 |
CTCCGAT | 240 | 0.0 | 101.64583 | 9 |
TCGCCCA | 160 | 0.0 | 100.40626 | 8 |
GTCTACC | 575 | 0.0 | 100.37392 | 7 |
TGTCTAC | 615 | 0.0 | 99.650406 | 6 |
TCTCCCG | 895 | 0.0 | 98.39105 | 8 |
TCTCCCC | 1335 | 0.0 | 98.05244 | 8 |