FastQCFastQC Report
Thu 6 Aug 2015
G118-M11_ACTGAT-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M11_ACTGAT-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560036
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA305692285.86772718028695No Hit
CTCTTTGTCTCCCATTAACCATACTTACCAGATCGGAAGAGCGTCGTGTA333620.9371253549121413Illumina Single End PCR Primer 1 (100% over 21bp)
CTCTTTGTCTCCCATTTTAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA52970.14879063020711028No Hit
CTCTTTGTCTCCCCTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA48360.13584132295291396No Hit
CTCTTTTTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA41990.11794824546718066No Hit
CTCTTTGTCTCCCGTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA39440.1107853965521697No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGCAGCA35990.10109448331421368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC3323050.0118.890787
TGTCTCC3336800.0118.868056
CTTTGTC3352350.0118.8633353
TCTTTGT3363800.0118.851432
TTGTCTC3350300.0118.847275
CTCCCAT3312750.0118.840159
TCTCCCA3314900.0118.829488
CTCTTTG3355250.0118.812021
TTTGTCT3364000.0118.6975564
GTCTCCT7550.0106.390737
TCTCGCA3100.0105.564518
GTATCCC4850.0104.278367
CTCCCGT8200.0102.3109749
TGTCTCG3500.0101.999996
CTCCGAT2400.0101.645839
TCGCCCA1600.0100.406268
GTCTACC5750.0100.373927
TGTCTAC6150.099.6504066
TCTCCCG8950.098.391058
TCTCCCC13350.098.052448