FastQCFastQC Report
Thu 6 Aug 2015
G118-M12_ATTCCT-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M12_ATTCCT-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423618
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAAGTATGGTTAATGGGAGACAAAGAGAGATCGGAAGAGCACACGTCT382290.8642021078673611Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA205480.4645066549598089No Hit
GGTAAGTATGGTTAATGATCCAAGAAAGAATTATCTCTTCTGTTTAGTTT189790.42903795038360004No Hit
GGTAAGTATGGTTAATGATCAATGTTCTATCCCAGACCCATCAATTGTGA176670.39937896988392757No Hit
GGTAAGTATGGTTAATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA168240.3803221706756777No Hit
GGTAAGTATGGTTAATGATCATAGGGCTCCCAATGGAGGAGCTAGAGAAA164000.3707372562459055No Hit
GGTAAGTATGGTTAATGATCCACAAAATCAGAAGGTACATAAAAGCATAT143060.32340043828377585No Hit
GGTAAGTATGGTTAATGATCAATTTCATTTTAGTCATCAACATTACATCA128110.28960457254672534No Hit
GGTAAGTATGGTTAATGATCACTTCTAAAACTTTAGAATTTATTGTTTTA114630.2591317785577326No Hit
GGTAAGTATGGTTAATGATCTGTGAGCACGGAGACTGTGTCAAGAATTAA112390.25406805017973977No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTAGAGAAA100070.22621754410077904No Hit
GGTAAGTATGGTTAATGATCCACTTAGATTTGACCTTAGTACATAAAAGC97780.2210407860714917No Hit
GGTAAGTATGGTTAATGATCCAGCAATACCTCTCCTGGGCATATATCCAG97650.22074690897812607No Hit
GGTAAGTATGGTTAATGATCCTCCGGTAGTACATAAAAGCATATTTGGGA89350.2019839868632418No Hit
GGTAAGTATGGTTAATGATCAGTACATAAAAGCATATTTGGGATGAACAA87560.1979375253468993No Hit
GGTAAGTATGGTTAATGATCACTCTTTCCACCCCATCTCCGGAGCCAGGA83430.188601276149975No Hit
GGTAAGTATGGTTAATGATCATGTCTGCACTGTACATAAAAGCATATTTG81890.18511996289010488No Hit
GGTAAGTATGGTTAATGATCATCTTATGGCCAGATTCAGGAGGCTTGGCT77330.17481165869204798No Hit
GGTAAGTATGGTTAATGATCAATACAGTCCTCTGCCTTTAAAGCAATAGG74250.16784903217230782No Hit
GGTAAGTATGGTTAATGATCCTGATTCAACTTTGGTACATAAAAGCATAT67320.15218312250289243No Hit
GGTAAGTATGGTTAATGATCAGTCCAAACTTCTTTCTGCATGTACATAAA67040.15155015645564332No Hit
GGTAAGTATGGTTAATGATCATTAGATACAGATTCAAAACCATAATGTGT66880.15118846157150098No Hit
GGTAAGTATGGTTAATGATCAAAATCTTAGGCTGGAATTCAAAGCAGGTG66650.15066852517554635No Hit
GGTAAGTATGGTTAATGATCCATCTGAAGCTTGGGTGTGGAAAGAACAGT64450.1456952205185891No Hit
GGTAAGTATGGTTAATGATCAACAAAACGTTGAGGGTAAGTCACAGTCAT60600.13699193736891388No Hit
GGTAAGTATGGTTAATGATCTTTTTACTGTCATTGTATAGTCTGTGGTAT60250.13620072980985248No Hit
GGTAAGTATGGTTAATGATCCACTACAGCACTTGGTAACATGCTCACTCA58460.13215426829350999No Hit
GGTAAGTATGGTTAATGATCTGGCTGTAATCATTTGGAGAGTGGGAATGA57210.1293285270111479No Hit
GGTAAGTATGGTTAATGATCACATATGGAATAGTCATAATCGAACCCATT56650.12806259491664967No Hit
GGTAAGTATGGTTAATGATCACTTCCAGTCATAGTCAAGATCTTAATGTG55170.12471691723833296No Hit
GGTAAGTATGGTTAATGATCTCATAGAAAAAAAGTCAAGGTGGATAGATA54970.12426479863315502No Hit
GGTAAGTATGGTTAATGATCAACTTTTCCAGAATCCACACACAAGCAGGT53620.12121299804820398No Hit
GGTAAGTATGGTTAATGATCGCATAGGTAGCTAAAGAGGTGGCAACCCAC53550.12105475653639171No Hit
GGTAAGTATGGTTAATGATCCCTGGATATGGCAGTCTCTACATGGTCCAT52800.11935931176697445No Hit
GGTAAGTATGGTTAATGATCCACCCCATAATCAGCATCCAAACGCTGACA52390.1184324686263597No Hit
GGTAAGTATGGTTAATGATCAAAGAATAATATATAAAACTATTTAAATCT51340.11605884594917554No Hit
GGTAAGTATGGTTAATGATCTAGCATAAAAAAAATCTTCCATAATTTTAT51220.11578757478606877No Hit
GGTAAGTATGGTTAATGATCCTACAGTACATAAAAGCATATTTGGGATGA50160.11339134617862573No Hit
GGTAAGTATGGTTAATGATCCTTAACCCCATGTATCCTCTCCTACCTTTG49910.1128261979221533No Hit
GGTAAGTATGGTTAATGATCACAGTACATAAAAGCATATTTGGGATGAAC49130.11106293536195938No Hit
GGTAAGTATGGTTAATGATCTTGACTCAGAACAATATATGTGTGCACACG48760.11022651594238018No Hit
GGTAAGTATGGTTAATGATCATGTTAAATATACATGTGTATATGCGTATG48430.10948052024383662No Hit
GGTAAGTATGGTTAATGATCAAGTGACCATAGGTGTGTGGGTTCATTTCT48330.10925446094124765No Hit
GGTAAGTATGGTTAATGATCTCAAAGCACTGTTATTAACTTGGTTGCAGG46850.10590878326293092No Hit
GGTAAGTATGGTTAATGATCATAGTATCCTAGTCCACAGGTTCTGCAGCA46380.10484630454076278No Hit
GGTAAGTATGGTTAATGATCTGCAACCCTATAGGTGGAACAACATTATGA46000.10398727919092472No Hit
GGTAAGTATGGTTAATGATCTCCTTTTGTTGTCGAATTGCTCTGGCTAAT44490.10057378372183132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATGG4066750.0118.520075
GTATGGT4223250.0118.500676
GGTAAGT3875700.0118.488841
TATGGTT4226200.0118.433457
AAGTATG3896450.0118.429374
TAAGTAT3878750.0118.429123
ATGGTTA4234050.0118.3515858
GTAAGTA3898400.0117.867262
TGGTTAA4265450.0117.4845359
GGTTAGT187800.0110.587591
TGTATGG181550.0107.9486855
TAGTATG198650.0106.322444
TTAGTAT196750.0105.862673
GTTAGTA201400.0103.388932
GGTATGT163150.0100.421
GGTACGT5350.096.881331
AAGTACG3250.084.209214
ATGTATG208500.079.612874
ACGGTTA4850.078.5141458
TACGGTT4700.078.4880457
Produced by FastQC (version 0.11.2)