FastQCFastQC Report
Thu 6 Aug 2015
G118-M12_ATTCCT-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M12_ATTCCT-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423618
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA387299487.55263225712527No Hit
CTCTTTGTCTCCCATTAACCATACTTACCAGATCGGAAGAGCGTCGTGTA422180.9543771636700998Illumina Single End PCR Primer 1 (100% over 21bp)
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTTGGCCTTGGCATGGAAGCA66360.15001295319803837No Hit
CTCTTTGTCTCCCATTTTAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA65370.14777496610240756No Hit
CTCTTTGTCTCCCCTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA54450.1230892902596924No Hit
CTCTTTGTCTCCCGTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA46450.10500454605257506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC4197900.0118.890867
CTTTGTC4231050.0118.8804553
TCTTTGT4244400.0118.859822
CTCTTTG4233600.0118.8538361
TTTGTCT4241650.0118.838684
TGTCTCC4213550.0118.837616
CTCCCAT4184900.0118.8293849
TTGTCTC4229900.0118.819955
TCTCCCA4189200.0118.805428
GTCTACC7850.0109.14657
CTCCCGT7800.0109.083339
GTCTCCG3550.0107.267617
GTCTCAC7600.0104.907897
TCTCGCA4000.0104.1258
TCTCCCG8500.0102.900018
GTCTCGC4050.0101.370387
TCTCCTA9150.0100.142088
TGTCTCG4650.099.806466
TGTCTAC9450.098.222226
TCTCCCC15600.097.641028
Produced by FastQC (version 0.11.2)