FastQCFastQC Report
Thu 6 Aug 2015
G118-M13_ATCACG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M13_ATCACG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22260371
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4184211.8796676838854123Illumina Single End PCR Primer 1 (100% over 50bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT294630.1323562846279606No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA258180.11598189446168711No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT225490.10129660462532272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA487900.055.1768246-47
CCGTATC500400.054.8508148-49
GCCGTAT505800.054.47107746-47
CGTATCA500400.054.428748-49
TCGCCGT497200.052.43346844-45
GTATCAT535300.050.65224550-51
GATCTCG649200.050.4356834-35
GAGCGTC1613900.049.8077139
ATCTCGG663150.049.3029434-35
GTCGCCG580100.049.01236344-45
TCTCGGT655150.048.7697436-37
GTGGTCG589600.048.7070440-41
GGTCGCC580300.048.47767642-43
CGGTGGT630150.048.1551938-39
AGAGCGT1679600.047.9869428
TAGATCT716550.046.9697432-33
TCGGTGG710850.046.66419638-39
CTCGGTG685850.046.28307336-37
AAGAGCG1770200.045.7426957
TGGTCGC628550.044.74687242-43
Produced by FastQC (version 0.11.2)