G118-M14_CGATGT-_AC6PMVANXX_L003_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G118-M14_CGATGT-_AC6PMVANXX_L003_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27740869
Sequences flagged as poor quality	0
Sequence length	125
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	610451	2.200547502675565	Illumina Single End PCR Primer 1 (100% over 50bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT	36686	0.13224531646791599	No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT	32948	0.11877061241304301	No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA	29569	0.1065900278754786	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG	28192	0.10162623240101096	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GAGCGTC	141160	80.60489	9
AGAGCGT	144140	79.12832	8
AAGAGCG	150015	76.33087	7
CGGAAGA	168490	67.56212	4
TCGGAAG	174485	65.78982	3
ATCGGAA	173410	65.7516	2
GATCGGA	175235	65.30111	1
GAAGAGC	191930	59.81311	6
CGCCGTA	66865	57.159687	46-47
CCGTATC	69285	56.610237	48-49
GCCGTAT	69650	56.360554	46-47
CGTATCA	69280	56.189198	48-49
GATCTCG	85915	55.355118	34-35
TCGCCGT	66865	55.282097	44-45
ATCTCGG	88225	53.865288	34-35
TCTCGGT	85990	53.542393	36-37
GTCGCCG	76835	52.944813	44-45
GTATCAT	72925	52.69942	50-51
TAGATCT	92555	52.537815	32-33
GGAAGAG	220005	52.388557	5