FastQCFastQC Report
Thu 6 Aug 2015
G118-M17_ACAGTG-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M17_ACAGTG-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25687682
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3509191.3660983501742197TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG468250.055.40015446-47
AGAGCAC997100.054.133148
CTCGTAT461600.054.0446742-43
GAGCACA1044750.051.7894679
CGTATGC507250.051.5154444-45
CGGAAGA1007600.051.2291954
GATCTCG457750.050.04742438-39
ATGCCGT527450.049.38518546-47
ATCGGAA1049050.048.9374282
GCCGTCT530800.048.7710548-49
ATCTCGT467100.048.5233840-41
GATCGGA1075600.047.7861981
TCGGAAG1092450.047.3145983
TCGTATG570600.046.3535142-43
AAGAGCA1167400.046.338147
AGTGATC489300.046.1532636-37
TGCCGTC568150.045.2297448-49
CCGTCTT571200.044.59714550-51
GAAGAGC1224350.044.323676
GTATGCC609500.043.51257344-45
Produced by FastQC (version 0.11.2)