FastQCFastQC Report
Thu 6 Aug 2015
G118-M18_GCCAAT-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M18_GCCAAT-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19421894
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1666670.8581397880144954TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG225900.052.6759246-47
TATCTCG212650.051.6351438-39
CTCGTAT231750.050.69144442-43
CGTATGC255600.047.03213544-45
CGCCAAT264750.045.47074532-33
ATCTCGT244450.044.8571840-41
GTCACGC274050.044.45973228-29
ATGCCGT270850.044.27436446-47
ACGCCAA270850.044.25991432-33
GCCGTCT274450.043.41206448-49
TCACGCC281000.042.97891630-31
TGCCGTC296650.040.04295348-49
CACGCCA306100.039.59075530-31
TCGTATG306400.039.5159942-43
AGTCACG312200.038.89344828-29
CCGTCTT304350.038.6871750-51
ATATCTC285900.038.1664638-39
CAATATC296950.036.9977236-37
GTATGCC329600.036.9601244-45
CCAATAT302300.036.91648534-35
Produced by FastQC (version 0.11.2)