FastQCFastQC Report
Thu 6 Aug 2015
G118-M19_CAGATC-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M19_CAGATC-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13017081
Sequences flagged as poor quality0
Sequence length125
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG712330.5472271394792734No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT557090.42796845160600905No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG306870.2357440965451471No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA270090.2074889139892423No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC255390.19619605962350545No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA244900.1881374172904048No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA242160.1860324906943423No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA240990.1851336716733959No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT213620.16410745235433352No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC196600.15103232437441236No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC195360.15007972985648627No Hit
ATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGC189750.1457700078842561No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGG186240.14307355082141687No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC185070.14217473180047047No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG180190.13842581143960003No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG178000.13674340660552084No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT171980.13211871386526672No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA157710.12115619469526231No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGC156350.12011141361108532No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG154760.11888994160826072Illumina Single End PCR Primer 1 (100% over 50bp)
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT151230.11617812011771303No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC150860.11589387820510604No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC148650.11419610894331839No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC148450.11404246466623354No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT147880.11360457847654172No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG147760.11351239191029079No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA146020.11217568669965255No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC140640.10804265564606996No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC138120.10610673775480078No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA137160.10536924522479348No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA136280.10469321040562012No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCG135790.10431678192676223No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA132400.10171251143017393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA128750.070.568161
TGCCAGT134700.068.157763
GCCAGTA137300.066.867084
CCAGTAG141850.064.931985
CTGCCAG148600.061.5821042
CAGTAGC150050.061.462856
TAGCATA163300.056.4758159
GTAGCAT164200.056.383688
GCGGCCT42650.053.7104347
TCACCCG156350.049.510393
AGTAGCA254400.037.023787
CGATCCA43250.036.59422338-39
CTCACCC218500.035.836162
GACACGG137950.035.75607314-15
GCATATG162400.035.4288810-11
CACCCGG219900.035.22924
GGCGATC46550.034.3834636-37
CGGACAG143850.034.22749318-19
ATCCACG47000.034.11755440-41
GCGATCC47350.033.3627338-39
Produced by FastQC (version 0.11.2)