FastQCFastQC Report
Thu 6 Aug 2015
G118-M2_AGTTCC-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M2_AGTTCC-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6005478
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG92257815.362274243615579No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA4886438.136621264785251No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT969291.614009742438487No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT683651.138377328166051No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC416670.693816545493964No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT338900.5643181108980834No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT297030.4945984316319201No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG268800.44759134909827325No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA168020.2797778961141811No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA149560.24903929379143508No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC147730.2459920759013687No Hit
GAAGCAGTAGCAGAGTGATCAATGGAATGCGGGCTGTGCCTTACTAAATG147720.24597542443748854No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG147440.24550918344884454No Hit
CTTACCATTTCACACAGATCATATAACAAGAATTTTACTTAAATAGTGAG100520.16738051492320843No Hit
ATATTTCAAGTGGCATGATCTGCAACCCTATAGGTGGAACAACATTATGA73040.12162229218057247No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA62750.1044879358479042No Hit
CTTACCATTTCACACAGATCATATCTGTGGATGTAAACTCTCCCCAAAGC60600.10090787111367322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTAG786600.0116.776134
AGGTTTA1060150.0116.7382055
AAGTGGC868500.0116.687588
GTATGGT713500.0116.6196756
GGTAAGT658500.0116.583581
GAGGTTT1048550.0116.4389344
GTTTAGA1100400.0116.336047
TATGGTT715650.0116.294267
CTCTTAG857250.0116.171458
AGTGGCA872550.0115.9823159
AGTAGCA832600.0115.909166
GTAGCAG832750.0115.9025657
TGAGGTT1054900.0115.8977363
ACACTGC818500.0115.761042
CATGAGG1056650.0115.685491
TTTGAGC819650.0115.62816
GCTCTTA862100.0115.58697
CAAGTGG876100.0115.532727
ATGGTTA721550.0115.5164958
TAGCAGA833950.0115.464698
Produced by FastQC (version 0.11.2)