FastQCFastQC Report
Thu 6 Aug 2015
G118-M3_ATGTCA-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M3_ATGTCA-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6306223
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG82056913.012051746346426No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA4401886.980216208656116No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT1040131.649370788188112No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT784691.2443105801999073No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC661191.0484722788902328No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC300150.47595843026800666No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA246120.3902811556140656No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG241350.3827171985513357No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT237700.37692926494987566No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT211570.3354940033043551No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA144310.2288374515141631No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA130100.20630415384930093No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG122100.19361827198308718No Hit
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCCACCATCTGAC111480.17677776380568844No Hit
CTTACCATTTCACACAGATCAAAAATTCAGGTGACAGCAGATGCTGGCGA100480.15933467623964456No Hit
CACACTGCTCTTAGAGGATCTCATGGAGGCATTTCCTCAAGGGAGGCTCC88500.1403375681449895No Hit
CACACTGCTCTTAGAGGATCTGCAACCCTATAGGTGGAACAACATTATGA87040.13802239470440547No Hit
CACACTGCTCTTAGAGGATCATTCCCAGCCTCCCAGAGGGTCACCATAGT86400.13700752415510836No Hit
CACACTGCTCTTAGAGGATCAAGCTGGTACAGATTCTGAGGTAGTTAGCA85510.1355962197974921No Hit
CACACTGCTCTTAGAGGATCCTCTGCTGGAAGCAAGTACAGATTCTGAGG85320.1352949301031695No Hit
ATATTTCAAGTGGCATGATCTTCAACCCTATAGGTGGAACAACATTATGA75260.11934243365640575No Hit
CACACTGCTCTTAGAGGATCCACCTGCCTCTGCTTCCCAAGTGCTGGGAT66960.106180831220209No Hit
ATATTTCAAGTGGCATGATCTCCTTTGGAATCTGCCCTTGGGCCCTTGCT65700.10418280482628034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT689900.0117.956051
GTTTAGA1029400.0117.020227
CAATCTT904450.0116.898961
GAGGTTT979800.0116.880954
GCAGTAG717750.0116.8471764
AGTAGCA756300.0116.636856
AGGTTTA997950.0116.4969945
TGAGCTC1005600.0116.458698
TTTGAGC1009850.0116.43996
CTCTTAG924600.0116.41658
AAGTGGC953500.0116.3948758
TATGGTT757000.0116.356087
ATGAGGT987500.0116.3051152
TGAGGTT989050.0116.29733
GAGCTCT1007700.0116.1805659
AAGTATG703400.0116.152084
TTGAGCT1010700.0116.141837
CATGAGG989550.0116.0702441
CAGTAGC741800.0116.05955
GGTTTAG1039550.0115.980686
Produced by FastQC (version 0.11.2)