FastQCFastQC Report
Thu 6 Aug 2015
G118-M4_CCGTCC-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M4_CCGTCC-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496360
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG57991212.89736586928093No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA3592937.990752519816029No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT640361.4241742209253707No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT510721.1358521114857352No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC414810.9225462374009199No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC231490.5148386695015524No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA215160.47852040317056466No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG185480.4125114537092226No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT154170.34287734967840655No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT136730.3040904198062433No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA127610.2838073463868552No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA111840.2487345319324965No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG97970.21788735777384374No Hit
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCCACCATCTGAC77550.17247284470104707No Hit
CACACTGCTCTTAGAGGATCTTCTCAGGCTCTTCAGGGACTAAGCATGCA60050.13355247355638783No Hit
CTTACCATTTCACACAGATCATCTGGAAGAGTCATTAGTGCTTTTAATGG52240.11618286791982849No Hit
GGTAAGTATGGTTAATGATCCTTGTGTTTGCGAAAAGCAAGTATTTGCTG50790.11295803716784245No Hit
CTTACCATTTCACACAGATCTAAGAGCCTGAGGTAGTAGATTGCTATAAG49750.11064505511124555No Hit
CACACTGCTCTTAGAGGATCTAAACAGTCCCTTACTTCCTAAAGAAATAG48460.1077760677525821No Hit
CACACTGCTCTTAGAGGATCTGATTGGTCTAGGTACAGATTCTGAGGTAG47990.10673077778469696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT546900.0117.5684051
GTATGGT599650.0117.1112066
GTTTAGA725850.0116.955357
AGGTTTA701650.0116.892145
AAGTGGC675050.0116.828198
AAGTATG553150.0116.814564
TATGGTT602400.0116.527217
GCAGTAG488650.0116.4547354
CAAGTGG678950.0116.244757
AGTGGCA677750.0116.239889
GAGGTTT696800.0115.964664
CTCTTAG680100.0115.794498
AATCTTT547250.0115.693132
GTAGCAG519350.0115.685557
GCTCTTA679700.0115.67887
GTAAGTA556650.0115.577752
TCTTAGA678400.0115.558449
AGTAGCA520850.0115.5237356
ATGGTTA608150.0115.5135048
CATGAGG698550.0115.4531
Produced by FastQC (version 0.11.2)