FastQCFastQC Report
Thu 6 Aug 2015
G118-M4_CCGTCC-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M4_CCGTCC-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496360
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG66736914.842428097394336No Hit
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC59403613.211486624736454No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT57009512.679033707265432No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA54084512.028507503847557No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA4298569.560088605004937No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT3803268.458530900550668No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC2063424.589089841560729No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG565531.257750713910808No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG269410.5991735537190083No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTTAAAGAT83750.18626177619229775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATC520100.0118.280469
GGTGTAT521200.0118.213488
TCAGTTA661200.0118.117993
CAGTTAC661500.0118.064424
GTTACAA662150.0118.047376
AGGTGTA522450.0117.907877
CCTTAGT675900.0117.821567
TTAGTAT675300.0117.767659
TTCAGGT527400.0117.725034
GAACCTT675150.0117.7043844
GTCAGTT664400.0117.477442
CTTAGTA676750.0117.4449848
AAGAACC672500.0117.398462
TTACAAT666950.0117.377517
GTCTCCC593500.0117.326967
AATTCTC730500.0117.240533
TAAATTC731900.0117.178851
TGGTTGA595400.0117.142429
AGTTACA666800.0117.134915
ATTCTCT732400.0117.123234
Produced by FastQC (version 0.11.2)